I've added five new populations to my previous dataset (SIT: Southern Italians, ESC: East Sicilians, SWS: Southwest Sicilians, NWS: Northwest Sicilians, CYP: Cyprus), taken from this recent paper. The resulting principal components plot again shows the J2/R1b/E3b "coastal" vs. I/R1a1 "continental" groupings along the first principal component (right vs. left respectively).
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To be more precise, you'd have to separate individual countries into regions. Northern and Southern Italy would be quite different, as would Dalmatian vs Pannonian Croats, or Northern vs southern Greeks.
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