I've added five new populations to my previous dataset (SIT: Southern Italians, ESC: East Sicilians, SWS: Southwest Sicilians, NWS: Northwest Sicilians, CYP: Cyprus), taken from this recent paper. The resulting principal components plot again shows the J2/R1b/E3b "coastal" vs. I/R1a1 "continental" groupings along the first principal component (right vs. left respectively).
To be more precise, you'd have to separate individual countries into regions. Northern and Southern Italy would be quite different, as would Dalmatian vs Pannonian Croats, or Northern vs southern Greeks.
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