The samples belonged to the Afanasyevo (which did not yield a result), Andronovo, Tagar, and Tachtyk cultures. The non-R1a1 individual belonged to haplogroup C(xC3) and the Andronovo culture; the 2 other Andronovo individuals belonged to R1a1. This is an interesting result which suggests the presence of an eastern element in steppe cultures that originated by all accounts in the west of the area in question.
Int J Legal Med. 2007 Nov;121(6):493-9.
First successful assay of Y-SNP typing by SNaPshot minisequencing on ancient DNA.
Bouakaze C, Keyser C, Amory S, Crubézy E, Ludes B.
In the present study, a set of 13 Y-chromosomal single nucleotide polymorphisms (Y-SNPs) selected for the identification of the most frequent Asian Y-haplogroups was included in an allele-specific primer extension assay. Single nucleotide polymorphism (SNP) genotyping was accomplished by co-amplification of these 13 DNA fragments within 2 multiplex PCRs followed by detection with 1 minisequencing reaction using the SNaPshottrade mark Multiplex kit and analysis of extension products by capillary electrophoresis. First developed on modern samples, the assay was optimized for the analysis of 11 ancient DNA (aDNA) samples from the Krasnoyarsk region (southern Siberia) that were dated from 5,500-1,800 years before present (YBP). SNP typing was successful for most of them, which were all assigned to Y-haplogroup R1a1 except one. These results show that SNPs are well-suited for the analysis of aged and degraded DNA samples. Moreover, we found that the SNaPshot minisequencing methodology is a convenient, robust, and efficient method for SNP typing. To our knowledge, this study reports the first successful investigation of Y-SNPs on aDNA samples. The potential use of Y-SNPs in both evolutionary and forensic fields is also discussed.